RhoA-GTP 小鼠單抗/活性抗體使用說(shuō)明書及介紹
GTP酶是GTP/GDP結(jié)合蛋白。被細(xì)胞表面受體(GPCR)激活后, 它能調(diào)節(jié)下游效應(yīng)器的功能。費(fèi)斯德生物抗體，它可以結(jié)合GTP、水解GTP，因此在激活的GTP結(jié)合狀態(tài)和非激活的GDP結(jié)合狀態(tài)之間切換。它具有內(nèi)在的GTP酶活性，可將GTP水解為GDP，從而使G蛋白失活。我們精心篩選的小鼠單克隆抗體能捕捉到與GTP結(jié)合的具有活性的GTP酶。它們已被用于通過(guò)免疫組織化學(xué)監(jiān)測(cè)細(xì)胞和組織中 G 蛋白的活化和分布。
| 描述: Anti-RhoA-GTP Mouse Monoclonal Antibody |
| 背景介紹: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction. |
| 免疫原: Recombinant full length protein of active RhoA |
| 經(jīng)過(guò)測(cè)試的應(yīng)用: IP, IHC,IF |
| 推薦稀釋比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 濃度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亞型: IgG2b |
| 純度: Purified from ascites |
| 防腐劑: No |
| 成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
| 種屬反應(yīng)性: Anti-RhoA monoclonal antibody recognizes active RhoA from vertebrates. |
| 儲(chǔ)存條件: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western Blot: IP/WB Analysis of Active RhoA Protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-RhoA-GTP monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26007). 產(chǎn)品說(shuō)明書和使用介紹： https://www.neweastwuhan.com/wp-content/uploads/2022/05/26904_RhoA-GTP.pdf |
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