Rab11-GTP 小鼠單抗實(shí)驗(yàn)使用及操作手冊(cè)
Product Description
Pioneering GTPase and Oncogene Product Development since 2010
Rab11-GTP
Catalog Number: 26919
Gene Symbol: RAB11
Description: Anti-Rab11-GTP Mouse Monoclonal Antibody
Background: Small GTPases of the Rab-family are crucial elements of the machinery that controls
membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.
Immunogen: Recombinant full length protein of active
Rab11
Tested Applications: IP, IHC
Recommended Dilutions:
IP: 1 g for 1~2 mg total cellular proteins.
Immunoprecipitation and Western blot:
IHC: 1:50~100 dilution
Concentration: 1 mg/ml
Host: Mouse
Clonality: Monoclonal
Isotype: IgG2a, IgG2b P
Purity: Purified from ascites
Format: Liquid
Preservative: No
2+2+
Constituents: PBS (without Mg and Ca ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti- Rab11-GTP antibody
recognizes active Rab11 from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze /
thaw cycles
Rab11 Activation Assay.
Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPS (lane right). Immunoprecipitation
was done with the anti-Rab11-GTP monoclonal antibody (Cat. No. 26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21157).
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC APPLICATIONS
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