MED64應用手冊：人類干細胞來源的心肌細胞應用

關鍵詞：
人類干細胞來源的心肌細胞 Human stem cell-derived cardiomyocytes (hSC-CMs) 搏動頻率 beat frequency; 場電位時程 field potential duration (FPD)
慢性藥物作用 chronotropic drug effects; 藥物篩選 drug screening

人類干細胞來源的心肌細胞是制藥公司和科研機構在研究中應用得比較多的樣本。由于人類干細胞來源的心肌細胞具有和原代人類心肌細胞一樣的遺傳背景，它們的藥理學特性應該非常相似，因此結合人類干細胞來源的心肌細胞與MED64多電極胞外記錄系統的平臺已成為了研究人類遺傳性心肌疾病模型和藥物篩選的主流。
只需要將hSC-CMs培養在MED記錄電極上，就可以從MED64系統中記錄和提取出基本的電生理參數，比如搏動頻率、以場電位時程等。另外，由于電極的非侵入特性，穩定的記錄可以持續非常長的時間，因此慢性藥物的效果也能夠在MED64系統上進行測量。不僅如此，MED64還可以使用任意電極輸出刺激來Pace hSC-CMs，因此可用來研究頻率依賴或使用依賴的藥物作用。

參考文獻：

1.Abnormal calcium handling properties underlie familial hypertrophic cardiomyopathy pathology in patient-specific induced pluripotent stem cells.
Lan F, Lee AS, Liang P, Sanchez-Freire V, Nguyen PK, Wang L, Han L, Yen M, Wang Y, Sun N, Abilez OJ,Hu S, Ebert AD, Navarrete EG, Simmons CS, Wheeler M, Pruitt B, Lewis R, Yamaguchi Y, Ashley EA, Bers
DM, Robbins RC, Longaker MT, Wu JC.
Cell Stem Cell. 12(1):101-13 (2013)

2.Patient-specific induced pluripotent stem cells as a model for familial dilated cardiomyopathy.
Sun N, Yazawa M, Liu J, Han L, Sanchez-Freire V, Abilez OJ, Navarrete EG, Hu S, Wang L, Lee A, Pavlovic A, Lin S, Chen R, Hajjar RJ, Snyder MP, Dolmetsch RE, Butte MJ, Ashley EA, Longaker MT, Robbins RC, Wu JC.
Sci Transl Med. 4:130 (2012)

3.A novel method of selecting human embryonic stem cell-derived cardiomyocyte clusters for assessment of potential to influence QT interval.
Yamazaki K, Hihara T, Taniguchi T, Kohmura N, Yoshinaga T, Ito M, Sawada K.
J Toxicol In Vitro. 26(2):335-342 (2011)

4.Usefulness of field potential as a marker of embryonic stem cell-derived cardiomyocytes, and endpoint analysis of embryonic stem cell test.
Koseki N, Deguchi J, Yamada T, Funabashi H, Seki T.
Toxicol Sci. 35(6):899-909 (2010)

5.Improvement of the embryonic stem cell test endpoint analysis by use of field potential detection.
Koseki N, Deguchi J, Yamada T, Funabashi H, Seki T.J Toxicol Sci. 35(5):619-29 (2010)

6.Progressive maturation in contracting cardiomyocytes derived from human embryonic stem cells: Qualitative effects on electrophysiological responses to drugs.
Otsuji TG, Minami I, Kurose Y, Yamauchi K, Tada M, Nakatsuji N.Stem Cell Res. 4(3):201-13 (2010)