建議：
1. 你可以看到，如果沒有自動(dòng)梯度儀，你必須每次分別測(cè)50～100個(gè)樣品的OD再繪成曲線根據(jù)峰位置確定CMV所在的收集管。如果你有日立DGF—U，再加一個(gè)帶流動(dòng)的分光光度計(jì)和分部收集器（國(guó)產(chǎn)）就可以組成完全自動(dòng)化的收集線。
2. 做試驗(yàn)前建議先看看我的文章（用戶培訓(xùn)的19篇中第2，3，3a及18篇等。搞清楚后再做實(shí)驗(yàn)。
3. 以上推薦的方法用于初試，確定了CMV在CSCL，Surcrose，Percoll中的浮密度準(zhǔn)確值后可用階梯梯度進(jìn)行容量較大的分離（過程簡(jiǎn)化）
4. 粗離：黃瓜花葉搗碎過濾后，高速離心機(jī)8x50ml轉(zhuǎn)頭，4，000rpm，5℃，20分，去沉淀，上清液濃縮后加緩沖液用于超離。

附：CsCl回收方法：
   Protocal of recovery of caesium chloride after use ：
1. Starting with about a litre of CsCl gradient solution，extract it six times with an equal volume of acetone.
2. Filter off the crystals and suck dry.
3. Separate the aqueous filtrate from the acetone and heat the remaining aqueous solution in a fume hood with stirring to reduce the volume to about 150 ml.
4. Allow the solution to cool and filter off a second crop of crystals.
5. Combine the first and second crop of crystals and heat them in a silica dish to 600℃ to burn  off all the organic material.
6. Dissolve the CsCl in a minimal amount (4-500ml) of distilled water and filter through Whatman No 54 filter paper to remove the carbon.
7. Concentrate the filtrate by heating in an evaporating dish and when a skin of crystals starts to form on the surface allow the solution to cool.
8. Filter off the crystals and concentrate the filtrate further by repeating Step 7.
9. Filter off a second crop of crystals，discard the filtrate，combine the two crops of crystals，and dry them at 130℃.